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Phylogenetics And Networks for Generalised HIV Epidemics in Africa

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Mochudi Prevention Project

The Mochudi Prevention Project (Mochudi study) implemented a comprehensive package of behavioral and biomedical interventions to 16-64-year-olds in Mochudi, a village in the south of Botswana about 37 km (23 mi) northeast of the national capital, Gaborone. This is an NIH-funded Methods for Prevention Packages Program (MP3) study (R01 AI083036; PI: M. Essex).

Estimates of HIV-1 incidence and prevalence over time were the major goals of the enhanced HTC campaign in the northeastern sector of Mochudi (estimated total population of about 15,000) performed from May 2010 to August 2013. The baseline and two follow-up enhanced HTC campaigns targeted residents of the Mochudi community in households. Eligible residents were asked to complete an individual questionnaire with socio-demographic and HIV-related information, including ART status and patterns of sexual behavior (e.g., whether sexual partner resides outside of Mochudi), and to donate a blood sample for a rapid HIV test and viral genotyping (if HIV positive). Testing for anti-HIV-1 antibodies was performed in households using National HIV Testing guidelines and two Botswana-approved rapid tests. Individuals reported to be on ART received treatment through the National ART program and initiated ART based on Botswana national guidelines (treatment of all adults with CD4≤350 cells/µL or WHO Stage III/IV). To identify seroconverters, the follow-up enhanced HTC campaigns targeted individuals who had tested HIV-negative in a previous campaign. Seroconverters were identified based on a paired HIV-negative and HIV-positive test. Viral genotyping was performed to identify potential viral networks and clusters by HIV sequence analysis. Mathematical models were developed and parameterized to reflect the epidemic in Mochudi.

Mochudi Prevention Project